84 
For cultivation experiments it is always necessary that some 
of the particular blood or lymph should be sent home. Lymph 
may be taken in ordinary vaccine tubes which have been 
previously thoroughly sterilized by a heat as near 150 degrees 
Cent, as possible. This may also be done by placing them in 
carbolic lotion, 1 in 20, for an hour, and afterwards in 
absolute alcohol. They must then be heated to a temperature 
of 120 degrees in a test-tube plugged with cotton wool, in an 
oven for at least an hour. For blood, this will not suffice, as 
when coagulation has ensued, it is almost impossible to get 
any of the contents out again. In such a case the blood 
should be taken with a vaccine tube, and blown into a small 
tube containing some sterilized Koch’s gelatine, which is then 
to be sealed up in the flame of a bunsen or spirit lamp. After 
being thus placed in the tube the gelatine should, by a slight 
heat, be melted, and the blood mixed up with it by shaking. 
It is hardly necessary to indicate that all such materials 
should be carefully labelled. 
A. E. 
