CONTENTS 
PART I 
PAGE 
Introduction. 3 
Chapter I. Apparatus. 5 
Chapter II. Reagents.18 
Killing and Fixing Agents.18 
Dehydrating Agents.33 
Formulas for Alcohols.35 
Clearing Agents.36 
Miscellaneous Reagents.39 
Chapter III. Stains and Staining.41 
The Haematoxylins.43 
The Carmines.52 
The Anilins.54 
Combination Stains.63 
Chapter IV. General Remarks on Staining.69 
Selection of a Stain.69 
Theories of Staining.70 
Practical Hints on Staining.73 
Chapter V. Temporary Mounts and Microchemical Tests . . 76 
Chapter VI. Freehand Sections.83 
Chapter VII. The Glycerin Method.96 
Chapter VIII. The Venetian Turpentine Method .... 101 
Chapter IX. The Paraffin Method.107 
Killing and Fixing.107 
Washing.109 
Hardening and Dehydrating.110 
Clearing.Ill 
The Transfer from Clearing Agent to Paraffin.112 
The Paraffin Bath.113 
Imbedding.114 
Cutting.116 
Fixing Sections to the Slide.118 
Removal of the Paraffin.120 
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