PREFACE TO THE THIRD EDITION 
The continued appreciation accorded to Methods in Plant His - 
tology has exhausted the second edition. Since that edition appeared, 
methods have become more and more exact, so that the present 
volume is practically a new book. The general arrangement of the 
subject-matter, and directions for collecting material and for secur¬ 
ing reproductive phases in the algae and fungi have been retained, 
and a chapter on “Photomicrographs and Lantern Slides” (chap, xii) 
has been added. 
Great improvements have been made in the paraffin method, so 
that sections are easily cut which were impossible ten years ago, 
while ten years of added experience with the Venetian turpentine 
method have made it possible to describe it so definitely that even 
the beginner should find no serious difficulty. 
The author is deeply indebted to his colleague, Dr. W. J. G. 
Land, for numerous suggestions and improvements covering the 
whole field of microtechnic. He is also greatly indebted to Dr. S. 
Yamanouchi for many improvements in the methods applicable to 
algae and mitotic figures. 
Corrections and suggestions will be heartily appreciated. 
Charles J. Chamberlain 
Chicago 
May, 1915 
