PREFACE TO THE SECOND EDITION 
It is gratifying to the author to learn that the kindly reception 
accorded to Methods in Plant Histology has exhausted the edition. 
Since the first edition appeared, a little more than four years ago, 
laboratory methods have been greatly improved, and systematic 
experiments have* made it possible to give much more definite 
directions for making preparations. 
In the present edition much more attention has been given to 
collecting material. Professor Kleb’s methods for securing various 
reproductive phases in the algae and fungi have been outlined in a 
practical way. Methods for growing other laboratory material are 
more complete than in the earlier edition. 
The paraffin method has been much improved, and the glycerin 
method has been almost entirely replaced by the Venetian turpentine 
method, to which a whole chapter is devoted. Other new chapters 
deal with michrochemical tests, freehand sections, special methods, 
and the use of the microscope. 
The author is deeply indebted to his colleague, Dr. W. J. G. 
Land, for numerous suggestions and improvements in methods. 
Corrections and suggestions will be heartily appreciated. 
Chicago 
July 1, 1905 
Charles J. Chamberlain 
VI 
