68 
METHODS IN PLANT HISTOLOGY 
Directions for staining in safranin and Delafield’s haematoxylin 
are given in the chapter on “ Freehand Sections” (chap. vi). 
Haidenhain’s Iron-Haematoxylin and Orange G.—This haema¬ 
toxylin is very satisfactory when used alone. A light staining in 
orange G, however, sometimes improves the mount. After the last 
washing in water, stain for about 30 seconds in orange G, or, if the 
orange is in clove oil, stain after dehydrating in absolute alcohol. 
Eosin, erythrosin, and nearly all plasma stains fail to increase the 
effect of a good stain in iron-haematoxylin. 
Combinations might be described almost without limit. Several 
more will be suggested in connection with the various groups of plants 
in Part II. 
We have not attempted to make the list of stains complete. It is 
better to master a few stains than to use many stains indifferently. 
A successful photographer once advised an amateur to stick to one 
brand of plate and one formula for developer. His hint might well 
have a wider application. If one really masters two or three good 
combinations, he is well prepared to develop methods for meeting 
special needs. 
