CHLOROPHYCEAE 
195 
In about 24 hours this not only fixes, but it dissolves the lime with 
which most species are coated. 
For paraffin sections select the tip of the plant, a piece about 
a centimeter in length. Sections of this may show, no,t only the large 
Fig. 45.— Chara: fixed in chromo-acetic acid and stained in safranin, gentian-violet, orange. 
A, longitudinal section of apex showing five nodes; c, corticating filaments; n, part of the frag¬ 
mented nucleus. B, note the different condition of the nodes and first branches. C, young antherid- 
ium, o, and young oogonium, o. X230. 
apical cell, but also various stages in the development of antheridia 
and oogonia (Fig. 45). For the development of the plant body from 
the apical cell and also for early stages in the development of oogonia 
and antheridia, the safranin, gentian-violet, orange combination is 
