PHAEOPHYCEAE 
201 
The zoospores from the sporangia germinate immediately, 
forming dioecious, filamentous gametophytes bearing antheridia and 
oogonia. The fertilized egg at once begins to develop into the 
Laminaria plant. So little is known about this stage that it would 
be worth while for those who are at the seashore to germinate the 
zoospores of any members of the Laminariaceae. 
For habit study, small specimens up to 45 cm. in length can be 
mounted upon paper. They stick well and seldom need to be secured 
by gummed paper. Larger specimens may be allowed to dry and 
may then be stored away in a box. When wanted for use, wet them 
under the tap, or, better, 
in salt water; after using, 
let them dry and return 
them to the box. Speci¬ 
mens will stand 4 to 5 
such soakings in fresh 
water; if a pint of salt is 
added to 3 or 4 gallons of 
water, the material may 
be soaked a dozen times 
before it passes its useful¬ 
ness. If material has 
been fixed in formalin, it 
may be washed in sea¬ 
water—not very thor¬ 
oughly, but enough to 
remove the pungent odor 
—and then soaked in 
equal parts of glycerin and water. Use only enough of the glycerin 
to make the specimens flexible, not enough to make them wet to 
handle. In this way, material of Laminaria , Macrocystis, Nereocystis, 
Postelsia, and other large forms can be kept in condition for demon¬ 
stration and will last for years without any attention. When not 
in use, they should be kept stored in a box. 
Cutleria. —This alga deserves a place in any course in morphology, 
if the course is thorough enough to permit the study of three members 
of the Phaeophyceae. These three should be Ectocarpus (or 
Pylaiella), Cutleria , and Fucus. Cutleria is not found on the Ameri¬ 
can coasts, but is abundant at Naples. The habits of gametophyte 
Fig. 48 .—Cutleria multifida: A, oogonia; B, an¬ 
theridia. Fixed in Flemming’s weaker solution, cut 3/i. 
and stained in iron-alum haematoxylin. X470. 
