RHODOPHYCEAE 
207 
remove the surface glycerin with filter paper, and then crush the fila¬ 
ments between two slides. There is scarcely any danger of crushing 
too much. A little of the crushed material, including the various 
stages, can be put into the melted glycerin jelly. Add a round cover, 
tap gently until the jelly comes just exactly to the edge of the cover. 
As soon as the jelly is cool, the mount may be sealed with balsam 
but we prefer to leave the mounts for a day or two before sealing. 
Such mounts would probably keep for a year or two without sealing 
(Fig. 51). 
Fig. 52 .—Polysiphonia fibrillosa: A, nearly mature cystocarp, showing the large cell formed 
by the fusion of several auxiliary cells with the pericentral cell—the carpospores are large and elon¬ 
gated; B, an antheridium—the term “ antheridium ” is more correctly applied to the structure shown 
in C, a, which cuts off one or more sperms, s; D, young tetraspores. Fixed in Flemming’s weaker 
solution, cut 3 n, and stained in iron-alum haematoxylin. A and B X240; C and D X780. 
We have mounted Nemalion in Venetian turpentine; but by 
this method the material becomes hard and behaves like cartilage, 
so that it cannot be crushed under a cover. However, it can be 
crushed on a piece of glass with a scalpel. 
Nemalion, stained in eosin, makes beautiful mounts, but they 
always fade. 
Polysiphonia.—This is a very difficult form to handle, but Dr. 
Yamanouchi has developed an adequate method, and, by following 
it, anyone should be able to get good preparations. 
For mounting in glycerin, glycerin jelly, or in Venetian turpentine, 
fix in 10 per cent formalin and stain in iron-haematoxylin. 
