BRYOPHYTES—MUSCI 
241 
Archegonia.—Since the necks of the archegonia are usually 
long and more or less curved, it is necessary, for habit work, to cut 
sections as thick as 20 or 30 ju in order to get a view of an arche- 
gonium in a single section (Fig. 735). Mayer’s albumen fixative is 
not likely to held such sections to the slide. Use Land’s fixative. 
Here, as in case of antheridia, it is better to use fresh material, 
Fig. 75 .—Funaria hygrometrica: A, longitudinal section of capsule; B, transverse section of 
capsule of about the same age as A —Delafield’s haematoxylin and erythrosin; 10 /x. The columella, 
archesporium, outer spore case, two layers of chlorophyll-bearing cells, and the beginning of the air 
spaces can be distinguished at this stage. X420. 
putting particularly good pieces into 10 per cent glycerin for glycerin 
jelly mounts. 
For the development of the archegonium, trim away the leaves 
which usually cover the cluster. Fix in chromo-acetic acid with a 
little osmic acid and cut 5 to 10 ^ thick. For a study of the ventral- 
canal cell and fertilization, sections should not be thicker than 5 /jl. 
There is a general impression that the antheridia and archegonia 
of Sphagnum are rare and hard to find. Dr. George Bryan, who made 
