A CLASS LIST OF PREPARATIONS 
323 
ASCOMYCETES 
31. Peziza. —Paraffin sections of young apothecia, 5 p or less; sections of 
older apothecia, 10 or 15 /x. Safranin, gentian-violet, orange. 
32. Aspergillus, Eurotium. —Stain in eosin and mount in Venetian turpentine. 
33. Penidllium. —Treat like Aspergillus. 
34. Erysiphe commune on Polygonum aviculare. —Strip the fungus from the 
leaf. Paraffin, 5 p or less. Safranin, gentian-violet, orange. 
35. Uncinula necator on Ampelopsis quinquefolia. —Stain in Magdala red 
and anilin blue. Mount whole in Venetian turpentine and break the 
perithecia under the cover. 
36. Xylaria. —Paraffin sections of younger stages. Delafield’s haematoxylin 
and erythrosin. Be sure that some section in each mount shows the 
opening of a perithecium. 
LICHENS 
37. Physcia stellaris. —Cut in paraffin, 5 p. Stain in cyanin and erythrosin. 
BASIDIOMYCETES 
38. Puccinia graminis. —Aecidium stage on barberry leaf. Uredospore 
and teleutospore stage on oats. Cut 3 p and stain in iron-haematoxylin. 
39. Coprinus micaceus. —Paraffin. Transverse sections of gills showing 
trama, paraphyses, basidia, and spores. To show the basidium with 
four spores, the sections should be 15 p thick. For development of the 
spores, cut 5 p or less. Safranin, gentian-violet, orange. Boletus, 
Hydnum, and Polyporus are treated in the same manner. 
BRYOPHYTES 
HEPATICAE 
40. Riccia natans. —Paraffin, 10 or 15 p. Delafield's haematoxylin. Arche- 
gonia, antheridia, and sporophytes imbedded in the gametophyte. 
41. Marchantia polymorpha. —Paraffin, 5 or 10 p. Archegonia, antheridia, 
and sporophytes. 
42. Anthoceros laevis. —Paraffin, 5 or 10 p. Longitudinal and transverse 
. sections of sporophyte. Safranin, gentian-violet, orange. 
43. Pellia epiphylla. —Paraffin, 5 or 10 p. Longitudinal sections of sporo¬ 
phyte attached to gametophyte. Safranin, gentian-violet, orange. 
44. Porella platyphylla.—Psirafim, 10 p. Delafield’s haematoxylin. Arche¬ 
gonia, antheridia, sporophyte, and apical cell. 
MUSCI 
45. Sphagnum. —Leaf buds. Cut 5 p and stain in safranin and anilin blue. 
46. Sphagnum. —Capsule. Paraffin. Delafield’s haematoxylin and ery¬ 
throsin. 
