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METHODS IN PLANT HISTOLOGY 
47. Funaria hygrometrica. —Paraffin. Longitudinal and transverse sections 
of young capsules. Delafield’s haematoxylin. 
48. Funaria hygrometrica or any favorable form. Protonema. Place the 
well-cleaned material directly into 50 per cent glycerin and allow it to 
concentrate. Mount in glycerin or glycerin jelly. 
49. Bryum. —Paraffin. Antheridia, 10 p; archegonia, 15 to 20 p; capsule, 
lO/i. 
PTERIDOPHYTES 
LYCOPODIALES 
50. Lycopodium lucidulum. —Transverse section of stem. Paraffin sections. 
Safranin and Delafield’s haematoxylin. 
51. Lycopodium inundatum. —Paraffin. Longitudinal sections of strobilus. 
52. Selaginella. —Paraffin. Longitudinal sections of rather mature strobili. 
Safranin, gentian-violet, orange. 
53. Isoetes echinospora. —Transverse section of stem. Paraffin. Safranin 
and Delafield’s haematoxylin. 
54. Isoetes echinospora. —Paraffin. Longitudinal sections of microsporangia 
and megasporangia. Safranin, gentian-violet, orange. 
EQUISETALES 
55. Equisetum arvense. —Prothallia in Venetian turpentine. Stem-tips in 
paraffin. Transverse section of stem freehand or in celloidin. 
FILICALES 
56. Botrychium virginianum. —Paraffin. Stain rhizome, stipes, and root 
in safranin and Delafield’s haematoxylin. Stain sporangia in iron- 
haematoxylin. 
57. Protostele. —Use Gleichenia. Cut freehand and stain in safranin and 
anilin blue. 
58. Solenostele (amphiphloic siphonostele). —Use Adiantum or Dicksonia 
punctilobula. 
59. Ectophloic siphonostele. —Use Osmunda cinnamomea. 
60. Polystele. —Use Pteris aquilina or any species of Polypodium. 
61. Sporangia. —For development, use Pteris, Aspidium, Cyrtomium, or 
try any available species. For mitosis, Osmunda is exceptionally good. 
62. Antheridia and archegonia. —Mount whole in Venetian turpentine. 
Iron-alum haematoxylin. Sections should be 5 to 10 p thick. Stain in 
iron-haematoxylin and orange. 
63. Embryo—Pteris and Adiantum are good. Cut longitudinal vertical 
sections 10 p thick. 
