A CLASS LIST OF PREPARATIONS 
325 
SPERMATOPHYTES 
GYM NOSPERMS 
CYCADALES 
64. Zamia. —Freehand sections of stem. Safranin and light green. Trans¬ 
verse sections of microsporophyll, 5 or 10/z. Longitudinal sections of 
entire ovule, 10 to 15 ^u; stain in safranin, gentian-violet, orange. 
Longitudinal sections of nucellus with pollen tubes, 10 /z. Iron-haema- 
toxylin and orange. 
GINKGOALES 
65. Ginkgo biloba. —Longitudinal sections of endosperm showing archegonia 
or young embryos. Paraffin 10 p. 
Sections of microsporangia with nearly mature pollen, 5 /z. 
CONIFERALES 
66. Pinus Laricio. —Transverse sections of needles and young stem. Free¬ 
hand. Safranin and light green. 
67. Pinus Strobus. —Freehand sections of well-seasoned wood. Safranin 
and Delafield’s haematoxylin. 
68. Pinus Laricio. —Paraffin. Longitudinal section of mature staminate 
strobilus. Safranin, gentian-violet, orange. 
69. Abies balsamea or Pinus Laricio. —Pollen at shedding stage shaken out 
and imbedded in paraffin; 5 p. Stain in safranin, gentian-violet, orange. 
70. Pinus Laricio. —Paraffin. Ovule with archegonia. Safranin, gentian- 
violet, orange. 
71. Pinus sylvestris or P. Laricio. —Paraffin. Embryos. Cyanin and ery- 
throsin, or safranin and anilin blue. 
GNETALES 
72. Transverse section of stem of Ephedra. Freehand. 
73. Longitudinal section of the ovule of Ephedra. 
A NGIO SPERM S 
DICOTYLS 
74. Pelargonium. —Transverse sections of stem to show phellogen and 
intrafascicular cambium. Freehand. Endarch siphonostele. 
75. Tilia americana. —Celloidin or freehand. Transverse sections of small 
stems 3 mm. to 6 mm. in diameter. Safranin and Delafield’s haematoxy¬ 
lin. Endarch siphonostele with annual rings. 
76. Sambucus nigra. —Transverse section of primary root to show origin 
of secondary structures. 
77. Cucurbita. —Longitudinal section of stem to show sieve tubes. 
