326 
METHODS IN PLANT HISTOLOGY 
78. Capsella bursa-pastor is. —Paraffin. Floral development, 5 p. Embryos, 
5 to 10 p. Stain both in Delafield’s haematoxylin without any contrast 
stain. 
79. Taraxacum officinale. —Paraffin. Floral development, 5 p. Embryo 
sac, 10 to 15 p. 
80. Ranunculus. —Longitudinal sections of young flowers to show megaspore 
mother-cells and megaspores. 
81. Silphium. —Longitudinal sections of the ovule at the fertilization period. 
Longitudinal sections of staminate flowers just before the shedding of 
pollen. 
82. Anemone patens. —Paraffin. Embryo sac. 
MONOCOTYLS 
83. Clintonia. —Transverse section of stem to show siphonostele. Paraffin. 
Safranin and anilin blue. 
84. Acorus calamus. —Transverse sections of rhizome, freehand or paraffin, 
to show amphivasal bundles. 
85. Zea Mays. —Transverse section of stem to show scattered bundles; also 
good for companion cells. Freehand. Safranin and anilin blue. 
86. Tradescantia virginica. —Longitudinal sections of root-tip. Paraffin, 
5 and 10 p. Stain for mitosis. 
87. Smilax herbacea. —Transverse section of adult root. Freehand. Shows 
exarch, radial structure, and a highly developed endodermis. Safranin 
and Delafield’s haematoxylin. 
88. Lilium. —Transverse section of leaf. Freehand. Transverse section 
of ovaries in various stages from megaspore mother-cell to fertilization; 
transverse sections of anthers to show microspore mother-cells and reduc¬ 
tion of chromosomes; also later stages with nearly mature pollen. 
Paraffin 5 to 10 p. 
89. Iris. —Section of young seeds to show embryo with cotyledon and 
stem-tip. 
90. Sagittaria. —Longitudinal sections of ovulate flowers of various stages 
to show development of the embryo and endosperm. 
91. Zea Mays. —Longitudinal and transverse sections of embryo (sweet corn, 
roasting-ear condition) to show structure of root and beginning of pro- 
toxylem. 
