328 
METHODS IN PLANT HISTOLOGY 
Schaffner’s Chromo-Acetic Solution.— 
Chromic acid. 0.3 g. 
Glacial acetic acid. 0.7 c.c. 
Water. 99.0 c.c. 
Chromo-Acetic Solution (for marine algae).— 
Chromic acid. 1-0 g. 
Glacial acetic acid. 0.4 c.c. 
Sea-water. 400.0 c.c. 
Material must be washed in sea-water. 
Strong Chromo-Acetic Solution.— 
Chromic acid. 1 g- 
Glacial acetic acid. 3 c.c. 
Water. 100 c.c. 
Licent’s Formula.— 
1 per cent chromic acid. 80 c.c. 
Glacial acetic acid. 5 c.c. 
Formalin. 15 c.c. 
Flemming’s Fluid (weaker solution).— 
f 1 per cent chromic acid (in water). 25 c.c. 
A \ 1 per cent glacial acetic acid (in water). 10 c.c. 
(Water. 55 c.c. 
B. 1 per cent osmic acid (in water).. 10 c.c. 
Keep the mixture A made up, and add B as the reagent is needed 
for use, since it does not keep well. 
Flemming’s Fluid (stronger solution).— 
1 per cent chromic acid. 45 c.c. 
2 per cent osmic acid. 12 c.c. 
Glacial acetic acid. 3 c.c. 
Special Chromo-Acetic-Osmic Solution.— 
Chromic acid. 1 g. 
Glacial acetic acid. 3 c.c. 
1 per cent osmic acid. 1 c.c. 
Water. 100 c.c. 
Good for algae (fresh-water), filamentous fungi, and many 
others; but not for root-tips, stem-tips, and many other things. 
The name, special, was selected because the formula is not general in 
its application. 
