FORMULAS FOR REAGENTS 
329 
Merkel’s Fluid.— 
1.4 per cent solution of chromic acid. 25 c.c. 
1.4 per cent solution of platinic chloride. 25 c.c. 
Benda’s Fluid.—* 
1 per cent chromic acid. 16 c.c. 
2 per cent osmic acid. 4 c.c. 
Glacial acetic acid. 2 drops 
Hermann’s Fluid.— 
1 per cent platinic chloride. 15 parts 
Glacial acetic acid. 1 part 
2 per cent osmic acid. 4 or 2 parts 
Picric Acid.—• 
Picric acid. 1 g. 
Water or 70 per cent alcohol. 100 c.c. 
Bouin’s Fluid.— 
Commercial formalin. 25 c.c. 
Picric acid (saturated solution in water). 75 c.c. 
Glacial acetic acid. 5 c.c. 
Corrosive Sublimate and Acetic Acid.— 
Corrosive sublimate. 3 g. 
Glacial acetic acid. 5 c.c. 
70 per cent alcohol (or water). 100 c.c. 
Corrosive Sublimate, Formalin, Acetic Acid.— 
Corrosive sublimate. 4 g. 
Formalin. 5 c.c. 
Glacial acetic acid. 5 c.c. 
Water (or 50 per cent alcohol). 100 c.c. 
For material to be mounted in Venetian turpentine, use the 
aqueous solution; for imbedding, use the alcoholic. Both this and 
the preceding solution should be used hot (85° C.). 
Bensley’s Formula (for mitochondria).— 
2\ per cent corrosive sublimate in water.4 parts 
2 per cent osmic acid. 1 part 
