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METHODS IN PLANT HISTOLOGY 
Corrosive Sublimate, Acetic Acid, and Picric Acid.— 
Corrosive sublimate. 5 g. 
Glacial acetic acid. 5 c.c. 
Picric acid.. • • 1 g- 
50 per cent alcohol.. 100 c.c. 
Corrosive Sublimate and Picric Acid (Jeffrey’s formula).— 
Corrosive sublimate, saturated solution in 30 
per cent alcohol. 3 parts 
Picric acid, saturated in 30 per cent alcohol. 1 part 
Iodine.—• 
To a saturated solution of potassium iodide in distilled water, add iodine 
to saturation. Filter and dilute with distilled water to a rich brown color. 
For fixing, dilute still farther to a light-brown color. Good for unicellular 
and filamentous algae. See directions in chapter ii. 
Gram’s Potassium Iodide Solution (for staining bacteria).— 
Iodine. 1 g- 
Potassium iodide. 2 g. 
Water. 300 c.c. 
Iodine (solution for starch test).—• 
Dissolve 1 -g. potassium iodide in 100 c.c. of water and add 0.3 g. 
iodine. 
Gilson’s Fluid.—• 
95 per cent alcohol. 42 c.c. 
Water. 60 c.c. 
Glacial acetic acid. 18 c.c. 
Concentrated nitric acid. 2 c.c. 
Corrosive sublimate (saturated solution in water) 11 c.c. 
Bensley’s Formula (for canal system).— 
1. Bichromate of potash. g. 
2. Corrosive sublimate.... 5 g. 
3. Water. 90 c.c. 
4. Formalin (neutral). 10 c.c. 
Make a solution of 1 , 2, 3, and then add the neutral formalin. 
Osmic Acid (stock solution).—• 
Osmic acid. 1 c.c. 
Distilled water. lc.c. 
The bottle in which the solution is to be kept, and also the glass 
tube in which the acid is sold, must be thoroughly cleaned. Break 
