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METHODS IN PLANT HISTOLOGY 
Anilin Blue.— 
Anilin blue.•. 1 g. 
85 or 90 per cent alcohol. 100 c.c. 
For staining before mounting in Venetian turpentine, this stain 
should be made up in strong alcohol, even if the dry stain is intended 
for aqueous solution. 
Iodine Green.— 
Iodine green. 1 g. 
70 per cent alcohol. 100 c.c. 
Methyl Green.— 
Methyl green. 1 g. 
Glacial acetic acid. 1 c.c. 
Water. 100 c.c. 
If the preparation is to be mounted in balsam, a slight trace of 
acetic acid and also a trace of methyl green should be added to the 
absolute alcohol used for dehydrating. 
For staining vascular bundles, the acid may be omitted, even from 
the formula. 
Light Green.— 
Light green. 1 g. 
Clove oil. 100 c.c. 
or 
Light green . 1 g. 
Clove oil. 75 c.c. 
Absolute alcohol. 25 c.c. 
Fuchsin.— 
Fuchsin. 1 g. 
95 per cent alcohol. 100 c.c. 
Water. 100 c.c. 
Acid Fuchsin.— 
Acid fuchsin. 1 g. 
Water. 100 c.c. 
Use this formula when staining woody tissues in methyl green 
and acid fuchsin. 
