FORMULAS FOR REAGENTS 
335 
Ziehl’s Carbol Fuchsin.— 
Fuchsin. 1 g. 
Carbolic-acid crystals. 5 g. 
95 per cent alcohol. 10 c.c. 
Water. 100 c.c. 
Fuchsin and Iodine Green Mixtures.—-Two solutions are kept 
separate, since they do not retain their efficiency long after they are 
mixed. 
. (Fuchsin (acid). 0.1 g. 
\ Distilled water. 50.0 c.c. 
-p. (Iodine green...,. 0.1 g. 
\ Distilled water. 50.0 c.c. 
( Absolute alcohol. 100.0 c.c. 
C | Glacial acetic acid. 1.0 c.c. 
[iodine. 0.1 g. 
Stain in equal parts of A and B. Transfer from the stain directly 
to solution C, and from C to xylol. 
Another Formula.— 
. (Acid fuchsin. 0.5 g. 
A \ Water. 100.0 c.c. 
(Iodine green. 0.5 g. 
\ Water. 100.0 c.c. 
Mix a pipette full of A with a pipette full of B; stain 2 to 8 min¬ 
utes; dehydrate rapidly and mount in balsam. 
Magdala Red.— 
Magdala red. 1 g- 
85 or 90 per cent alcohol. 100 c,c. 
Use formula when staining in Magdala red and anilin blue, 
before mounting in Venetian turpentine. Phloxine is the American 
equivalent. 
Safranin.— 
Safranin. * S* 
95 per cent alcohol. 50 c * c * 
Water. 50c * c * 
