336 
METHODS IN PLANT HISTOLOGY 
Safranin (another formula).— 
Dissolve 1 g. of alcohol-soluble safranin in 100 c.c. of absolute alcohol; 
dissolve 1 g. of water-soluble safranin in 100 c.c. of distilled water. Mix 
equal parts of the two solutions. 
Gentian-Violet.— 
Gentian-violet. 1 g. 
95 per cent alcohol.. 20 c.c. 
Water. 80 c.c. 
Anilin oil. 3 c.c. 
A 1 per cent solution in water keeps better. 
A 1 per cent solution in clove oil is worth a thorough trial. 
Pyoktanin.— -This is sold by E. Merck, in Darmstadt, Germany. 
Dissolve 1 g. of pyoctanin in 30 c.c. of water. 
Orange G.— 
Orange G. 1 g. 
Water. 100 c.c. 
For most purposes a 1 per cent solution in clove oil is preferable. 
It is easier to get a solution by dissolving 1 g. of orange in 100 c.c. 
of absolute alcohol; then add 100 c.c. of clove oil. Let the absolute 
alcohol evaporate until there is left about 100 c.c. of the solution. 
Gold Orange.— 
Gold orange . 1 g. 
Clove oil. 100 c.c. 
Bismarck Brown.—• 
Bismarck brown. 2 g. 
70 per cent alcohol. 100 c.c. 
Nigrosin.— 
Nigrosin . 1 g. 
Water. 100 c.c. 
Gram’s Solution.—■ 
Iodine . 1 g. 
Iodide of potassium. 2 g. 
Water. 300 c.c. 
