30 e ANNÉE 
JUILLET 1916 
N° 7 
ANNALES 
DE 
L’INSTITUT PASTEUR 
Mémoire publié à l’occasion du jubilé de E. Metchnikoff. 
SOIY1E OBSERVATIONS ON THE VIRUS OF VACCINÏA 
by EDNA S. HARDE 
(Bureau of Laboratories, Health Department, New York City.) 
The work to be recordecl on the virus of vaccinia falls under 
the folio wing three heads : 
1. Methods of obtaining a pure virus; 
2. Combination of the virus of vaccinia with living tissue 
cells in vitro (Harrison’s method); 
3. The resuit of incubating the virus in various media. 
Methods of obtaining a pure virus. 
Although in the commercially prepared vaccine the conta- 
minating organisms are comparalively negligible; a comple- 
tely purified virus is désirable for experimental work. 
With DoctorM. Grund, the chloroform method of Green 1 ; 
and the ether method of Fornet [2] were lesled on a number of 
viruses obtained from the calf and the rabbit. To examine the 
purity of the virus, inoculations were made on ordinary media, 
incubateJ aerobically and anaerobically, while its activity 
2t 
