608 
ANNALES DE L’INSTITUT PASTEUR 
These experiments were conducted with very great care as if 
theobjecthad been to ascertain the coagulation température of 
a proteid. (Tliis fact is of some importance in view of the sta- 
tements wbich hâve been made as to the léthal effect of a tem¬ 
pérature of 85°G.) 
These experiments were repeated in 1911 with naturally 
infected rnilk ohtained from the tuberculous udder of a cow. 
This milk was highly pathogenic when fresh, 0,2 gramme of it 
being capable of producing in 15 days a tuberculous ulcer at 
the seat of inoculation, well marked tuberculosis of the popli- 
teal, superficial and deep inguinal, sublumbar and rénal lym- 
phatic ganglia corresponding to the leg inoculated and distinct 
tuberculous lésions of the retrohepatic, bronchial, suprasca- 
pular* ganglia and of the spleen, liverand lungs. 
Some of lbat milk was divided inlo 2 parts, one of wbich 
was kept for control experiments and the other heated in a 
steam jacketted copper pan, provited witli a stirrer by means 
of wbich the milk was kept in constant motion, the tempéra¬ 
ture of the fluid was recorded by means of a well tested and 
contiolled recording thermometer during the experiment. In 
the course of 8 minutes the température rose from 58°C. to 
78°C., and was for 4 minutes ab >ve 68°G. À sample of tbat 
milk was collected as soon as the température had reached 
78°C. The same milk was heated for 10 minutes more, the 
température rising gradually to 92°C. when a second sample 
was ta ken. 
3 sets of cobayes were inoculated subcutaneously with the 
original milk and with the same milk after it had been heated 
to 78°G. and 92°G. respectively. The quantity of untreated 
milk inoculated was 1 ce. ; the quantity of treated milk was 
very much larger, the sédiment of 40 ccs. ohtained by centri- 
fugalisation being used. The animais inoculated with these 
sam pies were examined frequently during life and the lésions 
recognisable hy inspection and palpation noted. After the 
death of the animais they were dissected carefully and the 
various organs examined for tuberclc hacilli. 
The following summary gives the results of the observations 
made. 
