AN GUILLULIDiE. 
11 
“ None of these manurial applications when sown as a top-dressing 
appear to be able to kill the Sitona maggots, the millepedes, or the 
small white worms, each of these insects being found this day in 
plenty.”—J. J. W. 
From this it appears that sulphate of ammonia and sulphate of 
potash at the rate (together) of 4 cwt. per acre did best, for the disease 
entirely ceased, and the Clover made such a good growth that in the 
period between April 3rd and May 14tli not a blank was to be seen. 
In the case of the sulphate of iron, it is of interest to notice that, 
though the application at 2 cwt. per acre caused the Tylenchus -attack 
to cease, the application of half that amount did not entirely check the 
spread of the disease. 
With regard to the permanent effect of the above-named application 
to the Stem-sick Clover, I wrote to Mr. J. Willis, requesting his opinion 
on the subject, and was favoured by him with the following highly 
satisfactory reply:— 
“ Harpenden, Oct. 25th, 1889. The manurial applications tried in 
the TylencMis-iniestedi Clover-field at Botham stead appear to extend 
their beneficial effects to the second crop as well as the first. The 
vigour of plant is now (July 26tli) very marked on the portion which 
received 3 cwt. sulphate of potash and 1 cwt. sulphate of ammonia per 
acre ; the growth of plant being less luxuriant with the 2 cwt. sulphate 
of iron. On each of these portions of the field, however, the ravages 
of the Tylenchus have quite ceased. On the portion which received 
1 cwt. sulphate of iron only, some plants are even in the second crop 
dying off; so that the experiment clearly proves that a large amount 
of fertilizing material is required to enable the Clover-plants to with¬ 
stand or to overcome the baneful effects of the Tylenchus .”—J. J. W. 
Looking at amounts used in Mr. Elder’s experiments, and the 
almost parallel one of Mr. Willis, it will be seen that Mr. Elder applied 
altogether 3f cwt. of mixture, consisting of 3 cwt. (all but 14 fibs.) of 
sulphate of ammonia—the rest sulphate of potash and steamed bones ; 
whilst in the experiment No. 2 of Mr. Willis at Bothamstead, the 
application was at 4 cwt. per acre, of which 3 cwt. were sulphate of 
potash, and the remainder was sulphate of ammonia. But in both 
these instances, whether the ammonia or potash was the chief ingredient, 
good results followed immediately on application. 
It may bear on the subject of applications at once useful to Clover 
and hurtful to the Eelworms to observe that in the record of the 
Woburn experiments before quoted it is noted that “ Sulphate of potash 
has given the greatest yield; the addition to it of bone-dust and 
superphosphate with nitrogenous manures has given no appreciable 
advantage”; also in a table given by Dr. Bitzema Bos of effect of 
various chemical applications on Tylenchi, that of ammonia and also of 
