52 
I. NAGAI : 
active peroxidase system tlian the rest of the portion where the development 
of the antliocyanin pigment is inhibited, in spite that the cliromogenic 
substance can be detected in a slight extent. 
The above observation agrees with the findings of Iveeble and Armstrong 
(1912) 1 who have shown the parallelism extisting in the distribution of 
antliocyanin and peroxidase in the corolla of Primvla sinensis and in others. 
When the seed coat is still green just previous to the formation of 
antliocyanin, a distinct red colour develops at the portion of black patch by 
treating the bean with hydrochloric acid in cold. It shows that the antliocyanin 
pigment is already present in a colourless state. 
Oxidation seemed to accelerate the development of antliocyanin in the 
seed coat of the soy bean. Slightly coloured beans rapidly deepened in 
colour if the pod was opened and exposed to air. Injury also accelerated the 
development of the pigment. The portion near the injury became purple at 
first on exposure to air’. Conversely the exclusion of air retarded the develop¬ 
ment of the pigment. The slightly red-coloured beans ceased to develop the 
purple pigment when kept in a closed chamber in which the air was 
replaced by hydrogen gas. Similar phenomena were observed in ripening 
grapes. In both cases, light had no influence. 
(b) The Cross : Blue Tinged Yellow X Brown and 
the Reciprocal. 
The colour of the seed coat of the parental plants are the following : 
“ Tanishi.” Yellow with a tinge of blue colour which is most prominent 
around the liilum, fading gradually further beyond. 
“ Ilaiiro.” Brown with a tinge of greyish green colour. 
The F, seeds produced on the F L plant were green with a pale blue 
tinge as in “Tanishi” (see Plate I). In F, solid blacks, and non-tinged 
greens and yellows were found, together with the parental type, by the 
following numbers. 
1. Keeble, E. and Armstrong, E. F., The Kole of Oxydases in the Formation of the Antho- 
cyan Pigment of Plants. Jour. Gent. 2: 277, 1912. 
2. Keeble, F. and Armrtrong, E. F., Loc. cit. 
