A GENETICO-PHYSIOHOGICAL STUDY ON THE FORMATION ETC. 
71 
behavior of the chromogen containing types in the two species of plants is 
due to the presence and absence of an inhibitor. The authors showed that 
in the cross between buff and white, the F 1 seed was buff and segregated in 
F, buffs, reds and whites by a rate 9:3: 4. While the cross between deep 
buff and white gave deep buff in I\ (F z seed) and deep buffs, buffs, reds 
and whites in the F, generation by the ratio 27 : 9 : 12 : 16. Thus we see 
that the deep buff differs from buff by a single factor-di ff erence and buff and 
red also by another factor pair. We see therefore that the inhabitor is also 
present in that case but differs horn that of the soy bean in such a way 
that the inhibitor in the Adzuki-bean inhibits the formation of reddish brown 
pigment from the chromogenic substance but the action does not seem to 
extend over the formation of the chromogenic substance, while in the case of 
the soy bean, the inhibitor inhibits the formation of the chromogenic substance, 
so that no chromogenic reaction can be observed in the green and yellow. 
In the buffs, the chromogenic substance can readily be directed and if another 
gene, a chromophelein is added to it, the deep buff is produced. 
The waiter was able to test the chromogenic substance by the material 
w r hich was kindly furnished to him through the courtesy of Mr. Fukuyama. 
Fully ripened deep buff and buff were found to be rich in the chromogenic 
substance P. The green unripe beans born on the plants raised from the 
same material in the next year also gave the similar result. 
In order to compare the genotypic compositions of the types of Adzuki 
and those of the soy beans, it is convenient to change the designations used 
by the authors to those proposed in the present paper. They gave RHf for 
buff, Rif for red, and rhf for white in which R is the gene for red, II an 
inhibitor and F the gene for buff. We assume that the genes for the red 
pigment are G and o and by the action of an inhibitor / results in the 
formation of buff The gene I only inhibits the action of o but that of C is 
left free. We have some data for believing that the reddish brown pigment 
of the Adzuki-bean is the oxdidation product of the chromogenic substance. 
The pigment is insoluble in strong acids, but readily soluble in water and 
especially in weak alkalies yielding a deep w r ine red colour which becomes 
yellow by acid. The alkaline pigment is insoluble in ether and acetic ether 
but in a weak acid solution, sparingly soluble in ether, and by evaporating 
