72 
I. N AG AI : 
the solvent yields an amorphous reddish brown pigment. The pigment may 
be precipitated from aqueous solution by lead acetate. 
Consequently the gene I seems to inhibit the action of oxidizing agency 
acting on the chromogenic substance. The above mentioned relation which 
exists in buff and red seems to be analogous to that of the case of the 
dominant white in Primula sinensis. According to Keeble and Armstrong 
(1912)', Keeble, Armstrong and Jones (1913) 2 and Keeble and Miss 
Fellow (1910)" certain dominant whites contain chromogen, which occurs in 
the recessive "white in an extremely slight amount and the inhibitory substance 
which obscures the peroxidase reaction is present in the former. The buff 
coloured seed coat of the Adzuki-bean can be considered somewhat analogous 
to the dominant white in the flower of Primula and the white to the recessive 
white. The colour of buff and white in the seed coat differ slightly from 
each other. The peroxidase reaction in the seed coat was also examined and 
an indication to the similar relation that was observed in the flower of 
Primula sinensis was obtained. In the epidermis of the seed coat in which 
the pigment is confined, the peroxidase reaction was extremely slight in the 
unripe green seed of buff and deep buff whereas in the white, very distinct. 
The observation was repeatedly made with the material taken at the different 
stage of maturity. The section was placed under the cover glass with the 
alcoholic solution of benzidine or alpha naphtol with a dilute solution of 
hydrogen peroxide. In this manner, the direct oxidase has failed to be 
detected in all cases. 
The reddish brown in the soy bean (such as “ Aka-nedzumime ”) corre¬ 
sponds with red in the Adzuki-bean. The gene 0 which modifies the reddish 
brown to brown in the soy bean corresponds with the gene I in the other, 
inasmuch as they suppress the formation of reddish-brown, oxidation product 
of the chromogenic substance, though they differ in the manner toward the 
formation of the chromogenic substance as already mentioned. The genetic 
composition of the different self coloured types in both species can be expressed 
by the same designations in the following manner : 
1. Keeble, F. and Armstrong, E. F., Loc. cit. 
2. Keeble, F., Armstrong, E. F., and Jones, W. N., The Formation of the Anthocyan 
Pigments of Plants. 6. Proc. Roy. Soc. London, B. 87 :113, 1813. 
3. Keeble F. and Pellow, C., White Flowered Varieties of Primula sinensus. Jour. Gene¬ 
tics. 1:1, 1910. 
