46 
I. NAGAI : 
The chromogenic substance can be detected in the extract of the milk ripe, 
green grains of the reddish brown sort but not in that of the pale buff. 
Table 19. 
Chromogen content in the extract of the milk-ripe 
grain of Oryza sativa 1 . 
Name of variety 
Colour of fully 
matured grain 
Chromogen 
F F 
Colour of extract 
with peroxidase with KOH (boiled) 
“ Uhei ” 
Pale buff 
— 
— 
— 
Yellow 
“ Kinoshitamochi ” 
11 
+ 
— 
— 
» 
“ Kameno-o ” 
?? 
— 
— 
— 
19 
“ Togo ” 
?? 
— 
— 
— 
11 
“ Seld-yama ” 
?? 
— 
— 
— 
11 
“ Yamato-chikara ” 
?? 
— 
— 
— 
11 
“ Akamoro ” 
Beddish brown 
II 
— 
Orange brown 
Deep blue to red 2 
“ Haguro ” 
?? 
II 
— 
11 
11 
As we have already seen the cliromogen in the awn belongs to the group 
of the chromogenic substance F, and in the grain, to the chromogenic sub¬ 
stance P. 
According to Kondo (1917), 5 the reddish brown pigment of the grain is 
confined to a single cell layer of the integument of the seed coat. In the dark 
coloured grain of the foreign sort, the writer observed that the pigment was 
not confined to the seed coat. An anthocyanin-like pigment was found in the 
pericarp, namely the layer above the tube cells and even in the grain of the 
brown sort of Japanese rice, a yellowish pigment was found in the cell of 
the pericarp. 
Parnell et Ah have shown that in Indian rice, the red, grey brown, and 
1. Three grams of fresh material were extracted with 15 cc of a 40 per cent alcohol. 
2. The extract was boiled with caustic potash. A deep blue colour like that produced by 
the solution of myricetin was found. On cooling, the blue colour changed to red. It may in¬ 
cidentally be mentioned that most of the flavones give a deep yellow colour when treated with 
caustic potash, but myricetin gives a deep blue instead of yellow. 
3. Kondo, M., Untersuchung ueber die Dicke der Beiskleischicht. Bericht, d. Ohara Inst. f. 
Landwirt. Forsch. 1: 219, 1917. 
4. See also Kikkawa, S., On the Classification of Cidtivated Bice. Jour. Coll. Agri. Imp>er. 
Univ. Tokyo. 3 :11, 1912, Pap.nell et al. Loc. cit. 
