10 
I. NAGAI : 
Table 5. 
Influence of animal charcoal on the decolorization of 
anthocyanin by hydrogen peroxide. 
Charcoal added in gram. 
(3) 
No. of minutes required for 
decolorization ( t) 
qt 
0.05 
29.0 
1.45 
0.04 
34.5 
1.38 
0.03 
420 
1.26 
0.02 
62.0 
1.24 
0.015 
89.0 
1.34 
0.01 
140.0 
1.40 
Control 
ca. 400.0 
— 
When the concentration of hydrogen peroxide varied and the amount of 
animal charcoal made constant, at the temperature 20.0 C, the following result 
was obtained. 
Table 6. 
Same as Table 5. 
Charcoal added 
in gram 
Cc of hydrogen 
peroxide added 
Concentration equi¬ 
valent to oxygen 
in g. 
No. of minutes 
required for 
decolor. 
0.05 
•1 
1 =0.014 
6.0 
0.05 
1 
1/2 
11.5 
0.05 
1 
1/4 
18.5 
0.05 
1 
1/8 
20 5 
0 
1 
1=0.014 
ca. 180.0 
It seems clear, from the data so far presented, that although the oxidizing 
enzymes, are universally present in the plant cell, particularly co-existing with 
anthocyanin, and although the normal oxygen relation is essential to the 
formation of anthocyanin in the living tissue as we shall see later, yet these 
enzymes have no direct relation to the formation of anthocyanin from flavone. 
On the contrary, anthocyanin is converted to a flavone-like yellow substance 
by the action of the oxidizing enzymes. 
