6 
I. NAG AI : 
B. 
Time in minutes 
(!) 
Intensity of colour 
(a — x ) 
k 
0 
100.0 
0 
6.5 
97.50 
0.00101 
15.0 
90.00 
0.00679 
39.0 
75 00 
0.00738 
C. 
0 
100.0 
0 
6.0 
93.75 
0.01075 
15.0 
76.88 
0.01752 
26.5 
62.50 
0.01773 
48.0 
56.25 
0 01200 
62.0 
53.12 
0.01020. 
D. 
0 
100.0 
0 
5.0 
81.25 
0.04154 
14.0 
62.50 
0.03323 
18.5 
55.00 
0.03233 
36.0 
50.00 
0.02666 
30.5 
46.25 
0.02535 
415 
43 75 
0.01992 
The figures in the third column are the values of the velocity constant 
1 a 
calculated as unimolecular reaction, k =— log.--—. They show that the rate 
t a — x 
of decomposition in A was very slow owing to the relative high concentration 
of anthocyanin and the value of k increased as the reaction advanced. In B, 
a similar tendency was observed but in D, the value of k rapidly decreased 
as the reaction advanced. Since the strength of the enzyme was the same in 
all cases, the different values obtained indicate that the mode ol action of 
peroxidase differed by the relation of the initial concentration of the enzyme 
to the substrate. 
If the values of a—x in A are plotted against time, they show nearly a 
