A GENETICO-PH Y SIOLOGIC AL STUDY ON THE FORMATION ETC. 
To illustrate, the following results will be mentioned. Ten grams of the 
fresh petals of scaiiet Papciver Rhoeas 1 were extracted with 150 cc of distilled 
water. The colour of the extract was deep red. This was taken as 1Q0, and 
50, 25, and 12.5 per cent solutions were prepared. The peroxidase used was 
prepared from the pressed sap of hypocotyls and rootlets of the soy bean 
seedlings by precipitating with alcohol. Five cc of the enzyme solution was 
added to fifty cc of a dilute hydrogen peroxide solution (0.1 cc corresponded 
to about 0.0003 g oxygen). The mixtures were put in large test tubes of the 
diameter 4 cm and they were kept in the water bath. The temperature was 
kept between 17.0 and 17.5 C. At different intervals of time, five cc of the 
mixture were pipetted out into a test tube and one cc of concentrated hydro¬ 
chloric acid was added to stop the enzyme activity and to deepen the colour. 
The standard solution was prepared in the same manner immediately after 
the enzyme was added. Each portion was then compared witn the standard 
solution so prepared by Dubosq’s colorimeter and the intensity of colour of the 
different portions was expressed as percentages of the initial colour which was 
taken as 100. 2 They gave the following result. 
Table 1. 
Decolorization of anthocyanin by peroxidase. 
A. 
Relative 
strength of anthocyanin = 100 
B. 
yy 
„ „ „ = 50.0 
C. 
yy 
= 25.0 
D. 
yy 
= 12.5 
A. 
Time in minutes 
(0 
Intensity of colour 
( a—x ) 
fc 
0 
100.0 
0 
7.0 
100.0 
0 
40.0 
99.06 
0.00024 
63.5 
93.75 
0.00102 
1. According to Willstätter and Weil (Liebig. Ann. 412:231, 1916.) one of the pigments 
of a double purple-scarlet variety is mekocyanin which is a diglucoside of cyanidin. 
2. The initial colour corresponded with somewhere between Peach red to Scarlet in Ridqway’s 
Color Standard. 
