U 
On the Reserve Protein in Plants, II. 
BY 
G. Daikuhara, Nogakushi. 
I have given the results of my investigation on the occur¬ 
rence of active albumen in a large number of plants in Vol. II. 
No. 2 of the Bulletin of this College. As all the objects then 
examined were collected in spring, it seemed to me of interest to 
examine them during the fall. We know that an extensive 
transportation of nutritive materials takes place from the leaves 
to the stem, roots, and bulbs. I supposed therefore that leaves 
found rich in active albumen in spring might not show this kind 
of reserve material in autumn. On the other hand, it seemed 
also possible that plants of rapid growth and yielding none of 
the reactions of active albumen in summer might yet accumulate 
some active albumen late in autumn when development stops. 
I wanted also to test the leaves of evergreen plants in the 
fall, as well as a number of fruits. Accordingly, all the objects 
here mentioned were examined during the months of October, 
November, and December. The general result of this examina¬ 
tion has been that objects not showing any active albumen as 
reserve-material in spring also show none in autumn ; (I) and that 
most objects yielding a positive result in spring, yield the same 
in autumn, although usually to a much less extent. 
I have also repeatedly examined leaves partially dead, as 
shown by their brown and yellow spots, and could never produce 
proteosomes with caffeine in the brown parts, but always in the 
still healthy and green cells, even at points very close to the 
dead portions. In a few cases I could see, without caffeine 
treatment, bright globules in the cells, which in some in- 
(i) As examples of those’which give no reaction in spring, and do give one in 
autumn, I will mention the leaves of Deutzia Sieboldiana, Diospyros kaki , and Salix 
japonica. It should be mentioned here that sometimes the caffeine reaction does not 
set in immediately, especially in the case of thick cell walls, and can then be 
detected only after the prolonged action of a saturated caffeine solution. 
