ON THE RESERVE PROTEIN IN PLANTS. II. 
IQI 
distinguished by treatment with 1 p.m. ammonia, by which the 
forms of true proteosomes are not changed, (I) while the tonoplast 
loses its globular shape. (2) 
Another reagent for distinguishing the two kinds of globules 
is iodine solution, which produces vacuoles in the true proteo¬ 
somes, while it only causes the tonoplasts to shrivel up. 
The results of my examinations are shown in the following 
tables : 
RANUNCULACEÆ. 
Species. 
Objects 
Active 
Remarks. 
tested. 
ALBUMEN. 
Pæonia albiflora, Pall. 
Old leaves 
Much 
Even present in those 
living cells that 
Anemone japonica, Sieb.) 
et Zucc.j 
Flowers 
None 
were near brown 
dead portions of 
the leaves. 
y y yy 
Leaves 
None 
BERBERIDEÆ. 
Nandina domestica, Thunb. 
Old leaves 
Present 
yy • y 
Epid. of fruit 
Moderate 
TERNSTRŒMIACEÆ. 
Camellia japonica, L. 
yy yy 
(Epid. of old) 
( leaves 
Midribs 
Little 
Very much 
Leaves suffering from 
albinism also gave 
the reaction. 
yy yy 
Epid. of fruit 
yy 
yy 
Camellia Sasanqua, Thunb. 
Epid. of fruit 
Very much 
y y yy yy 
Leaves 
yy 
y y 
yy yy yy 
Flowers 
yy 
yy 
Camellia theifera, Griff. 
yy yy 
Epid. of fruit 
Flowers 
Much 
Moderate 
Small fat globules 
present. 
Ternstrœmia japonica, ) 
Thunb. j 
Leaves 
Moderate 
Also present in the 
reddish portion of 
the leaves. 
(1) They merely solidiy and seem to shrink a little. 
(2) In some cases, however, it is well, after the ammoniacal treatment, to apply 
10 0/0 acetic acid under the microscope, which does not affect the solidified proteo¬ 
somes, but destroys the globular shape of the tonoplast. I applied this treatment in 
the case of the petals of Ipomcea hedracea. 
