204 
kinoshita; asparagine in nelumbo. 
crystals, on treating it again with hot alcohol and evaporating. 
An approximate determination showed that this root contains 
asparagine amounting to nearly 2 % of the dry matter. 
The purified crystals showed not only the same crystalline 
forms as asparagine but also yielded up one molecule of water 
when dried at ioo°, while the nitrogen determination according 
to the method of Kjeldahl also agreed with that for asparagine. 
For the determination of nitrogen, I took 0.3 gr. of the substance 
dried at ioo° and obtained 0.06232 gr. nitrogen, that is 20.78 %. 
Calculated. Observed. 
Water of crystallisation . 12.11 % 12.14 % 
Nitrogen . 21.02 % 20.78 % 
The solubility in water nearly agrees with the number 
given by Guarcschi, thus : 
i part of asparagine dissolves 
in 55.86 pts of water at 10.5 0 
( Guareschi ). 
i part of the substance dis¬ 
solved in 56.72 pts of water at 
11 . 2 ° 
