212 
M. INOUYE ; 
ing to the formation of lactic acid, when the separation of casein 
takes place exactly as in the souring of milk. The lactic acid 
was determined by shaking out the filtrate repeatedly with ether, 
evaporating the ether, and boiling the syrupy sour residue with 
some water and finely suspended oxide of zinc. The filtrate was 
evaporated to a small volume and after a few days the crystal¬ 
lised lactate of zinc was collected, dried, and weighed. 
Thus, loo cc. of the milky liquid, that had been left for two 
weeks in contact with air, yielded 0.13 grm. of lactate of zinc, 
corresponding to 0,092 grm. of lactic acid. 
I also analysed the fresh milky liquid with the following 
results :— 
Soya bean milk. 
Cow’s milk. 
Water .. 
. 9 2 53 % 
86.08 % 
Albuminoids . 
. 3-°2 % 
4-00 % 
T?q fi 
JL CA t < • « ••• 1 t 1 ••• 
. 2.13 % 
3-°5 % 
Fibre . 
. 0.03 % 
— 
* \ sh ... ... ... ... 
. 0.41 % 
0.70' % 
Non-nitrogenous extract, 
includ- 
ing carbohydrates 
. 1.88 % 
— 
Milk sugar .. 
. — 
5.00 % 
The fat contained in this liquid as well as in the /o/w-tablets 
was found to consist partly of lecithin. Tofu dried at ioo° 
yielded 26.65 % fat an d 4*83 gr. of this fat yielded, after igniting 
with carbonate of soda and nitrate of potash in the usual way, 
0.280 grm. of magnesium pyrophosphate, which, multiplied by 
the lecithin-factor, 7.2703, corresponds to 2.035 grm. lecithin, 
amounting to 11.2 % of dried iofu , leaving for the genuine fat 
15.4 %; (l) more of the latter, therefore, is left in the refuse than 
of the former. 
In the manufacture of tofu -tablets from the freshly prepared 
milky liquid, about 2 % of concentrated brine as it is obtained as 
mother liquor from the preparation of sea salt, is added with 
constant stirring, whereupon a flocculent precipitate is soon 
formed which is separated by means of a cloth filter, slowly 
(1) A portion of this lecithin was probably present in the soya bean as lecith- 
albumin ; comp. Leo Liebermann , J. B. f. Thierchemie, 1893, p. 32, and E. Schulze, 
Chemiker Zeitg. 1894, Nr. 43. 
