4io 
SUZUKI ; 
a, o. i% solution of ammonium nitrate. 
b, ,, ,, ,, ammonium chloride. 
c, 0.2 % ,, ,, sodium nitrate. 
* 
d, distilled water. 
Time :—8 days. (Oct. 27—Nov. 4.) 
Temperature :—Min. io.5°C. ; Max. 40°C. 
Neither the solutions of sodium nitrate, nor the plants grown 
in the different solutions, gave any reaction for ammonia ; only a 
few leaves withered during the experiments, and these were re¬ 
moved. The result of the analysis was as follows. 
Table I :—In 100 parts of dry matter : 
Plants in 
Originale) plants 
Control^) plants 
Ammonium 
Ammonium 
Sodium 
(Oct. 27th) 
(Nov. 4th) 
nitrate 
chloride 
nitrate 
Asparagine nitrogen 0.14 
0.29 
0.78 
0.99 
0.39 
AsparagineO) 0.64 
1.38 
3-67 
4.67 
1.85 
This result shows that ammonium chloride produced much 
more asparagine than ammonium nitrate, and this again double as 
much as sodium nitrate, while an increase of asparagine in the 
control case, compared with the original plants, taken from the 
field, must evidently be due to the gradual transformation of 
nitrates that had been stored up in the stem and roots, whose 
presence had been shown by the diphenyl-amine test. 
tv' II. Experiments with yellow-lupine (Lupinus luteus). 
Young plants 20 c,m ‘ high were taken from the field ; 4—6 
plants were placed in 300 e c - of the following solutions :— 
a, 
0.1 % 
solution of 
urea. 
b, 
y y 
y y y y 
ammonium phosphate. 
c, 
0.2% 
y y y y 
sodium nitrate. 
d, 
distilled water. 
and kept in the glass house for 6 days, from Nov. 7th to 13th. 
Temperature :—Min. 8°C. ; Max. 35°C. 
After drying, the entire plants were used for analysis. 
Table II :—In 100 parts of dry matter. 
(1) “Original” means the plants which were analyzed at the beginning of the 
experimen ts,. 
(2) “ Control ” means the plants kept in distilled water. 
(3) The water of crystallization of asparagine is not included in these calculations. 
