412 
SUZUKI ; 
of the second with o. i% solution of sodium nitrate, while those 
of the third served as a control case. Ten days after the treatment, 
the plants were removed from the seed bed, (1) 2 3 dried, and only 
the stems and roots used for analysis, because it has been found by 
others that cotyledons and leaves always store up less asparagine 
than stems and roots. 
Average height of the control plants (2) 1 3*3 c ' ni ' 
,, ,, plants in ammonium nitrate io. i c,m * 
,, ,, plants in sodium nitrate 6.3 e - 111 - 
Average fresh weight of control plants 2.36 grams. 
,, ,, ,, of the plant in ammonium 
nitrate 1.79 grams. 
,, ,, ,, of the plant in sodium 
nitrate 1.20 grams. 
The total ammonium nitrate solution applied was 318 CC * = 
0.0557 gram nitrogen. 
The total sodium nitrate solution applied was 322 c,c ’ = 
0.053 gram nitrogen. 
The aqueous extract of a portion of these plants did not show 
a trace of ammonia, while the reaction for nitrates was obtained 
in the plants in ammonium nitrate and sodium nitrate with Knop's 
solution. 
Table IV :—In 100 parts of dry matter free from ash. 
Plants in 
Control plants 
"Ammonium nitrate 
Sodium nitrate 
Albuminoid nitrogen 
2.49 
2.58 
2-53 
Proteids 
15-59 
16.13 
I 5-83 
Asparagine nitrogen 
1.38 
3-94 
3-33 
Asparagine 
6.53 
i 8.57 
15.69(3) 
The same experiment was repeated later in the autumn with 
shoots a little more developed than in the first case. 
(1) Some of the plants had been attacked by a fungus, Fusarium Lateritium, especi¬ 
ally in the lower parts of the stems, and in the cotyledons; such plants were, of course,, 
discarded. 
(2) The better growth of the control plants in this case is evidently due to the very 
early stage of the shoots, which contained a large quantity of soluble nutrients from the 
cotyledons. Thus an increase of soluble nutrients had a retarding effect. In a 10 °/ 0 
cane sugar solution, e.g , young plants will not develope so well as in one of 1 °/ 0 only. 
(3) The cause of the considerable production of asparagine from nitrates may in 
this case be due to the high temperature, favouring the reduction of the nitrates by the 
increased amount of sugar present. 
