4 i6 Suzuki ; 
Table X :—In too parts of total nitrogen :— 
Plants in 
Original 
plants. 
Control 
plants. 
SugarO) 
Urea(U 
Sodium 
nitrate. 
Sodium nitrate 
and sugar. 
Total nitrogen 100 
100 
100 
100 
100 
100 
Albuminoid nitrogen 57.3 
56.5 
53 - 
53 -t 
54-6 
59-2 
Asparagine nitrogen 17.5 
21.3 
27.4 
45 -k 
22.4 
18 4 
Nitrogen in nitrates 0 
0 
0 
0 
6.0 
5-2 
We observe here no considerable decrease of albuminoid ni¬ 
trogen during the experiments, but a little increase of asparagine 
nitrogen takes place in the control and sugar case ; but as this 
increase does not correspond with the decrease of albuminoid 
nitrogen, it must be due to the transformation of other amido- 
compounds into asparagine. We observe here also a very great 
difference of asparagine nitrogen in the urea and sodium nitrate 
plants. Sodium nitrate does not here seem well suited to be¬ 
come asparagine. Sugar had no great influence upon the 
increase of albuminoid nitrogen ; this must be due to the want 
of some other necessary conditions for the protein formation 
(want of sulphates ?). 
Isolation of asparagine crystals : ß # 27 srams air dry plants 
treated with urea yielded o. i §ram by crystallization, while the 
calculation requires about 0.27 sram . In this case, I could not 
separate the slimy mother liquor which prevented the cry¬ 
stallization of all the asparagine. 
VII. Experiments with “ key as agar a ” (Holesia hispiduni). 
Young branches covered with leaves, 25-30 cm * in length, 
were placed in the following solutions :— 
a , o. i% solution of urea. 
b , ,, ,, ,, ,, and 10% cane sugar. 
c , 0.1% solution of ammonium phosphate, 
d, ,, ,, ,, ,, ,, and 10% cane 
sugar. 
e , distilled water. 
The objects were kept in the dark, for 7 days (Oct. 31st— 
Nov. 7th). No ammonia was found stored up after the treat¬ 
ment and the leaves remained generally very healthy. 
After drying, the leaves only were subjected to analysis:— 
(1) As the total nitrogen in some of the above cases was increased absolutely, the 
albuminoid nitrogen was apparently decreased. 
