FORMATION OF ASPARAGINE IN PLANTS. 
417 
Table XI :—In 100 parts of dry matter : — 
Plants in 
Original 
Control 
Amm. 
Amm. phosphate 
Urea. 
Urea and 
plants. 
plants. 
phosph. 
and sugar. 
sugar. 
Asparagine nitrogen 
O.I I 
0.15 
0.16 
0.10 
0.23 
0.12 
Asparagine 
°-5 1 
0.72 
o -73 
0.48 
1.10 
0-55 
In this case it is very evident that the introduction of sup-ar 
prevented the increase of asparagine. (1) 
VIII. Experiments with buckwheat plants ( Polygonum 
fagopyrmn). 
Experiments were made with buckwheat plants taken from 
the farm in full blossom and bearing seeds. There was only a 
trace of asparagine and a moderate amount of nitrate present, 
but no trace of ammonia. Preliminary experiments showed further 
that in this case, some of the ammonia offered as ammonium 
nitrate in 0.05^ solution, was found afterwards as such in the 
stems to the extent of o. i?% C2) of the air dry matter. 
The plants were now placed in the following solutions :— 
a, 0.1 % solution of ammonium nitrate. 
by 0.1% solution of ammonium chloride. 
c, 0.2 % solution of sodium nitrate. 
d, distilled water. 
The plants were kept in the glass house for 9 days (Oct. 
12 th—21st.). 
Temperature :—Min. i6°c ; Max. 40°c. 
All the plants were healthy and the solutions remained 
clear. 
The analysis for which only stems and leaves were used 
yielded the following results :— 
Table XII. In 100 parts of the fresh plants :— 
Plants treated with 
Control 
plants. 
Ammonium 
chloride. 
Ammonium 
nitrate. 
Sodium. 
nitrate. 
Asparagine nitrogen and 
nitrogen in ammonia. 
0.05 
0.13 
00 
0 
0 
0.06 
Ixitrogen in ammonia. 
0 
0 08 
0.04 
0 
Asparagine nitrogen. 
0.05 
005 
0.04 
0 06 
Asparagine 
0.25 
0 22 
0.18 
0.29 
(1) A small amount of sulphates was found in the leaves which might have been 
utilized in the production of new proteids from asparagine and sugar. 
(2) Cf. Analytical data in the appendix. 
