4i8 
SUZUKI ; 
The reason why so little asparagine was formed here and 
the ammonia taken up remained as such partly preserved in the 
plant, may be due to the fact that the development of young 
seeds required most of the sugar produced by the leaves, for 
the production of starch, and therefore the necessary amount 
of sugar for the production of asparagine was not available. In 
the following experiments, therefore, all the flowers and seeds 
were removed before the treatment commenced ; the plants 
were placed in the following solutions ;— 
a, 
b , 
c , 
d, 
e, 
/, 
o.\% solution of ammonium nitrate. 
,, ,, ,, ,, and io% sugrr. 
,, ,, ,, chloride and io% sugar. 
,, ,, ,, carbonate. 
,, ,, ,, ,, and \o% sugar. 
,, ,, ,, phosphate. 
,, ,, ,, ,, and \o% sugar. 
These were kept in the glass house for u days (Oct. 24th— 
Nov. 4th). 
Temperature:—Min. io.'5°c.; Max. 37°c. 
In this case, the result differed considerably, no trace of am¬ 
monia being found in any of them. 
The analysis for which only the stems and roots were used, 
yielded the following results. 
Table XIII:— 
Asparagine nitrogen. 
Asparagine. 
Plants treated w 
ith 
Amm. 
phos¬ 
phate 
and 
sugar. 
N 
. Ammo- Amrao- 
Aramo- 
. mum m- mum 
mum m-. , , , 
, , träte and carbon- 
trate. . 
sugar. ate. 
Amm. 
carbon¬ 
ate and 
sugar. 
Ammo¬ 
nium 
phos¬ 
phate. 
Amm. 
chloride 
and 
sugar. 
0.39 O.IO 
0.48 
0.27 
0.26 
1.10 
O.IO 
1.82 0.48 
2.25 
1.27 
1.20 
0.46 
0.50 
We see from these results, that the formation of asparagine 
from ammonia was considerable. The sugar produced from the 
leaves evidently yielded the carbon for the asparagine but 
we see also on the other hand, that, in those cases where sugar 
was added to the solutions, the amount of asparagine was again 
less, which finds a natural explanation in the fact that, by a larger 
excess of sugar, the protein production was so stimulated that 
