FORMATION OF ASPARAGINE IN PLANTS. 
419 
the asparagine was again for the greater part consumed. (1) Sugar 
in the nourishing solution therefore not only prevents asparagine 
production from proteids, as Monteverde has ascertained with 
Syringa but also lowers the amount of asparagine produced 
from ammonium salts offered to the roots ! 
IX. ( A .) Experiments with “ Mikawashinia-na ” 
(. Brassica campes tris). 
This experiment was made on the field, the plants about 
30 e111 - high with as yet very small roots were irrigated with the fol¬ 
lowing solutions :— 
a. , 0.1% solution of ammonium phosphate. 
b , 0.2% ,, ,, sodium nitrate. 
Duration of experiments, 8 days (Nov. 20th—Nov. 28th). 
Total solutions applied for each case, 600 c.c. 
The entire plants were used for analysis. 
Table XIV. In 100 parts of dry matter : — 
Control 
plants. 
Asparagine nitrogen 0.85 
Asparagine . 3.99 
(Z>.) Experimets with Brassica Campestris , var, 
“ Siuedish turnip." 
Full grown plants with large round roots, were removed 
from the farm, and placed in the following solutions :— 
a, 0.1% solution of ammonium nitrate. 
b y 0.02% ,, ,, ammonium carbonate. 
c, 0.2% ,, ,, sodium nitrate. 
d, distilled water. 
The plants were kept in the glass house. 
Time of experiments : — 7 days (Oct. 16th—Oct. 23rd). 
Temperature :—Min. I7°c ; Max. 40°c. 
At the end of the experiments, the plants had suffered very 
much, the temperature being too high, and a portion of the roots 
died. After washing the roots and removing the dried leaves, 
only the healthy parts were analyzed which yielded the following 
results :— 
(i) The presence of sulphates, which I found in the leaves and stems, no doubt, 
rendered this process possible. 
Plants in 
Ammonium 
phosphate. 
o -55 
2.60 
Sodium 
nitrate. 
0.83 
3-90 
