FORMATION OF ASPARAGINE IN PLANTS. 
421 
a , 0.2 % solution of ammonium phosphate. 
b, 0.4 % ,, ,, sodium nitrate. 
Time of experiments, Nov. 23rd-28th. 
Total solution applied : 
Ammonium phosphate 200 cc. 
Sodium nitrate 200 cc. 
After drying, the entire plants were analyzed. 
Table XVII. In 100 parts of dry matter :— 
Plants treated with 
Control plants Ammonium phosphate Sodium nitrate 
Asparagine nitrogen 0.39 0.47 0.42 
Asparagine 1.85 2.21 2.00 
II. Experiment. 
The plants were carefully removed from the field and placed 
in glass cylinders, each containing 500 c - c - of the following solu¬ 
tions, and kept in the glass house : 
a, 0.2^ solution of ammonium chloride. 
b, ,, ,, ,, sodium nitrate. 
c, distilled water. 
Duration of experiment : 7 days (Nov. 30th—Dec. 7th). 
Temperature :— Min. 3°C. ; Max. 33°C. 
Total nitrogen absorbed by ;— 
a, the plants in ammonium chloride was 0.0462 grams = 
°- 5 % °f dry matter. (1) 
b, the plants in sodium nitrate was 0.0437 g rams = 0 -59% 
of dry matter. (2) 
The plants remained normal and the solutions quite clear. 
The analysis, for which the entire plants were used, yielded the 
following results :— 
Table XVIII. In 100 parts of dry matter :— 
Plants treated with 
Total nitrogen 
Original 
plants 
4.60 
Control 
plants 
4 44 
Ammonium 
chloride 
483 
Sodium 
nitrate 
4.78 
Albuminoid nitrogen 
1.65 
1.63 
1.86 
i -35 
Asparagine nitrogen^ 3 ) 
0.28 
0.71 
0.86 
0.95 
Nitrogen in nitrates 
1.04 
0.36 
0.62 
0.88 
(1) Total dry matter of the plants in ammonium chloride (at the end of the ex¬ 
periments) was 9.328 grams. 
(2) Total dry matter of the plants in sodium nitrate (at the end of the experiments) 
was 7-47 grams. 
(3) As I had suspected the presence of some organic bases like arginine, etc , I em¬ 
ployed first phospho-tungstic acid to remove them. Indeed, the result for asparagine was 
then considerably lower than by the usual method, while in most of the other cases, I 
found no essential difference by the previous application of phospho-tungstic acid. 
