426 
Suzuki ; 
of dilute dammonia. The concentrated solution yielded an abund¬ 
ant quantity of large asparagine crystals which were washed 
with water and then with absolute alcohol, and dried over sul¬ 
phuric acid. The amount thus obtained was 0.51 gram, 1(1) the an¬ 
alysis proved the crystals to be pure asparagine. 
Calculated Observed 
Nitrogen . 18.66 % 18.45 % 
Water of crystallization... 12.0 % 12.2 % 
(lost at ioo°C.) 
XI. Experiment Avith barley (Hordeum distichon). 
Young barley plants of an average hight of 32 e ™- were removed 
from field and placed (in the glass house) in the following solu- 
♦ 
tions :— 
a. 
0.2 % 
urea. • 
b. 
y y 
,, and 2 % sugar. 
c. 
y y 
ammonium chloride. 
d. 
y y 
,, ,, and 2 
% sugar 
e. 
O Q/ 
2 A) 
sugar. 
f. 
distilled water. 
Duration of experiment :—7 days (March 26th—April 2nd). 
Temperature :—Min. io°C. ; Max 45°C. 
After drying, the entire plants f2) were analyzed with the 
following result :— 
Table XXIV. In 100 parts of dry matter : — 
Plants treated with 
^ ■ ■ ---—-- — 
Original 
Control 
Sugar 
Urea 
Urea and 
Amm. 
Amm. chloride 
plants 
plants 
sugar 
chloride 
and sugar 
Total nitrogen 
5-13 
5.08 
4.60 
5 65 
4.98 
5-73 
5.00 
Alb. nitrogen 
2.16 
1.86 
1.47 
1.50 
1.28 
1.60 
i 23 
Asp. nitrogen 
o-93 
1.84 
1.94 
i.87(?) 
2.15 
3.21 
2.09 
Nitrate nitrogen 
0.58 
0.37 
0.35 
0.49 
0.24 
0.28 
0.23 
Table XXV. : 
In 100 parts of total nitrogen :— 
Plants treated with 
Original 
Control 
Sugar 
Urea 
Urea and 
Amm. 
Amm. chloride 
plants 
plants 
sugar 
chloride 
and sugar 
Total nitrogen 
100 
100 
100 
100 
100 
100 
100 
Alb. nitrogen 
42.3 
36.6 
32 
26.6 
25-7 
27.9 
24.6 
Asp. nitrogen 
18. i 
36.2 
42.2 
33* i(?) 43-2 
55-8 
41.8 
Nitrate nitrogen 
11.4 
7-3 
7.6 
8.6 
4-8 
4-9 
4.6 
(1) A moderate quantity of asparagine was left in the mother liquor, which was not 
considered. 
(2) Toward the end of the experiments some leaves or tips of leaves began to dry 
up, owing to the high temperature. Such leaves were, of course, removed. 
