FORMATION OF ASPARAGINE IN PLANTS. 
429 
ANALYTICAL DATA. 
For the determination of total nitrogen cited in the fore¬ 
going pages 0.5 or i.o sram of air dry sample was mixed with 30 c c * 
sulphuric acid, i gram sugar, 2 grams benzoic acid, i gram mercury 
and o.5 gram copper sulphate, and decomposed and distilled as 
usual. 
For the determination of proteid nitrogen, Stutzer' s method 
was used. 
For the determination of asparagine nitrogen Sacchsse's 
method was generally used, but in some cases, when the presence 
of organic bases was suspected, phospho-tungstic acid was pre¬ 
viously applied. 
For the determination of nitrates, Tiemann and Schnitze' s 
method was used. 
HELIANTHUS ANNUUS. 
ASPARAGINE NITROGEN. 
Plants in 
Ammonium nitrate 
Ammonium chloride 
Sodium nitrate 
Control plants 
Original plants 
Dry matter 
used in 
grams. 
BarytaU) 
water re¬ 
placed in c.c. 
Nitrogen 
found in 
gram. 
j I- 4-535 
18.8 
0.0178 
(2. 
18.6 
0.0176 
( I- 4485 
23-7 
0.0224 
( 2. 
23-4 
0.0221 
( i- 4.550 
9-4 
0.0089 
( 2. 
94 
0.0066 
j i. 4-5*5 
7.0 
0.0064 
(2. 
6.8 
0.0030 
LO 
O 
4 
• 
3-2 
0.0029 
(2. 
3-1 
0.0029 
Percentage 
of nitrogen 
( = i asp. 
nitrogen.) 
0-39 
0.50 
0.20 
0.15 
0.07 
(i) 10 c.c. of the standard sulphuric acid were equivalent to 48 c.c. baryta solution, 
and yielded 0.374 gram barium sulphate. Hence ic.c. of the baryta solution = 0.0009447 
gram nitrogen. 
