FORMATION OF ASPARAGINE IN PLANTS. 
437 
HOLESIA HISPIDUM. 
ASPARAGINE NITROGEN. 
Plants in 
Ammonium phosphate 
Ammonium phosphate 
and sugar . 
Urea . 
Urea and sugar. 
Control plants . 
Original plants. 
Dry matter 
used. 
Gram. 
1. 3.664 
2. „ 
1. 3724 
2. ,, 
i. 3.668 
2 * j> 
1. I.79O 
2 . ,, 
1. 3.692 
2. „ 
I. 2 . 6 oI 
Baryta watert 1 ) 
replaced. 
c.c. 
3-o 
3 - 0 
1.9 
2.1 
4.2 
4- 5 
1.0 
1.2 
3 -o 
3 -o 
i-5 
1.6 
Nitrogen 
found. 
Gram. 
0.0028 
0.0019 
0.0042 
0.0010 
0.0028 
0.0015 
Percentage of 
nitrogen (J asp. 
nitrogen.) 
0.08 
0.05 
0.11 
0.06 
0.08 
0.06 
BUCKWHEAT. (I.) 
DETERMINATION OF AMMONIA. 
One gram of air dry matter of the ammonium nitrate plant 
(lower stems and roots) was extracted with warm water and to 
the filtrate, to which so much sodium bicarbonate added as 
necessary to render the liquid slightly alkaline, yielded on 
distillation 0.0017 gram nitrogen (=i.8cc. baryta solution (1) J 
=0.17^ nitrogen. 
Air dry samples were mixed with some caustic lime and 
moistened with water. The mixture was kept under a bell jar. 
The ammonia liberated was absorbed by a known quantity of 
standard sulphuric acid ; thus I obtained the following results: — 
i gram of air dry sample of the control plants yielded 
0.0004 gram nitrogen (=o.4CC. baryta solution (1) )= 0 . 04 / %' nitrogen. 
(i) I c.c . of the baryta solution = 0.0009447 gram nitrogen. 
