FORMATION OF ASPARAGINE IN PLANTS. 
441 
NITRATE NITROGEN. 
Dry matter 
used. 
Pressure. 
Tempera¬ 
ture. 
Volume of 
NO gas. 
Nitrogen 
found. 
Percentage 
of 
nitrogen. 
Gram. 
Inch. 
°c. 
c. c. 
Gram. 
( I- 0.913 
30.36 
9 
11.9 
) 
Control plants ... 
> 0.0076 
0.83 
(2. „ 
99 
99 
12.1 
) 
( i. 0.917 
99 
99 
6.0 
) 
Original plants... 
>• 0.0038 
0.41 
(2. „ 
99 
99 
6.0 
) 
BRASSICA. (TAINA). (I.) 
ASPARAGINE NITROGEN. 
Plants in 
Dry matter 
used. 
Baryta waterC 1 ) 
replaced. 
Nitrogen 
found. 
Percentage of 
nitrogen 
(J asparagine 
nitrogen.) 
Gram. 
c. c. 
Gram. 
Ammonium phosphate 
1.840 
2.0 
0.0043 
0 
to 
Sodium nitrate. 
1.830 
1.8 
0.0039 
0.21 
Control plants . 
1.858 
1-7 
0.0037 
0.20 
BRASSICA. (TAINA). (II.) 
I. Nitrogen determination of ammonium chloride solution 
applied to the plant (before experiments). 5 C,C - of the ammonium 
chloride solution were diluted with water to 250 c,c ’, and from 
this 20 c,c - and ioo c,c- were distilled after addition of soda solu¬ 
tions. 
1. 20 cc ’ yielded 0.0160 gram nitrogen ( = i 6 . 9 c,c - baryta 
solution (1) ) = o.o8% nitrogen. 
2. ioo cc - yielded 0.0792 gram nitrogen ( = 83.8 C,C - baryta 
solution) =0.08^ nitrogen. 
This calculated to the original solution gives 
1. 3 - 99 ,% 
2. 3 - 90 %- Average 3.98%. 
(i) I c.c. baryta solution = 0.0009447 gram nitrogen. 
