FORMATION OF ASPARAGINE IN PLANTS. 
445 
BRASSICA CAMPESTRIS VAR. TAINA. (III.) 
I. Examination of ammonium chloride solution that remained 
unabsorbed. The total solutions were diluted to 500 c,c ‘, of 
which 200 c,c ’ and ioo CCl were analyzed. 
(a) . 200 c,c ‘ of the ammonium chloride solution yielded 
0.0627 g ram nitrogen (=66. 3 e - 0, baryta solution). 
Hence total nitrogen in total solution=o.i568 gram. 
(b) . ioo c - c> of the ammonium chloride and sugar solution yielded 
0.0321 gram nitrogen ( = 34 c - c# baryta solution). 
• < 
Hence total nitrogen in total solution = 0.1606 gram. 
Total nitrogen originally applied = 0.1988 gram. 
Therefore, 0.1988 — 0.1568 = 0.042 gram nitrogen was absorbed by 
ammonium chloride plants. 
0.1958 —0.1606=0.0382 gram nitrogen was absorbed 
by ammonium chloride and sugar plants. 
II. Examination of sodium nitrate solution that remained 
unabsorbed. The total solutions were concentrated to ioo c,c ‘, 
from which 20 and io c,c - were analyzed. 
(a) . 20 c,c * of sodium nitrate yielded 
0.0218 gram (temp. I5°C., pressure 29.66 inch., vol. of 
NO 36.4 ;°- c -). 
Total nitrogen in total solution = 0.1090 gram. 
(b) . I0 C C ' of sodium nitrate and sugar solution yielded 
0.0113 gram nitrogen (temp. I5°C ; pressure 30 inches ; vol. 
of NO 18.5 C C * ). 
Total nitrogen in total solution=0.1126 gram. 
Total nitrogen originally applied = 0.1499 §T am nitrogen. 
0.1499 — 0.1090=0.0409 gram nitrogen was absorbed by 
sodium nitrate plants. 
0.1499—0.1126=0.0373 gram nitrogen was absorbed by 
sodium nitrate and sugar plants. 
