NAKAMURA ; ON ASPARAGINE AS A NUTRIENT FOR FUNGI. 469 
TABLE I. 
Nourishing material 
in grams. 
for 500 c. c. 
* 
Weight of fungus 
Ammonium tartrate 
6-33 
4 - 
ethyl alcohol 3.06 
0.015 
33 
malate 
5 -°° 
+ 
33 
33 
33 
O.OI I 
33 
succinate 5 00 
4 - 
r 
33 
33 
0.008 
33 
lactate 
7-13 
4 - 
33 
33 
33 
0.006 
33 
acetate 
5-13 
4 - 
33 
33 
33 
0.000 
33 
nitrate 
2.66 
4 - 
33 
S3 
33 
0.002 
Asparagine 
01 
b 
0 
4 - 
33 
33 
3* 
0.026 
33 
0 
0 
in 
alone 
0.016 
Second experiment. 
Here methyl alcohol was applied in addition to the nitro¬ 
genous materials which were asparagine, glycocoll, urea, beta'in, 
ammonium chloride, sodium nitrate, ammonium tartrate, am¬ 
monium malate, and ammonium succinate. All the solutions 
contained 1 °/o methyl alcohol, while the sources of nitrogen were 
applied in such proportion that the number of nitrogen atoms to 
those of carbon showed the ratio of 1 : 8. This time the amount 
of monopotassium phosphate was increased to 0.5 °/o mid to all the 
flasks ferrous sulphate, (1) 2 sodium sulphate, and magnesium sulphate 
(o. i % of each) were added. All other conditions were the same 
as before except that the volume of the solutions was only 200 c.c. 
and the temperature somewhat higher, ranging from 9 0 — I9°C. The 
flasks, after two weeks, exhibited a considerable difference; in be- 
taïn, ammonium succinate and ammonium malate, no fungoid de¬ 
velopment was yet noticed®; in ammonium tartrate only a moder¬ 
ate quantity was observed, much however in asparagine and glyco¬ 
coll. The flasks had been frequently shaken in order to prevent 
the formation of spores on the surface which would have again 
given rise to a great increase of the mycelium. Only during the 
last 4 days shaking was dispensed with, allowing now spores to 
(1) Certain fungi are only developed in presence of iron salts ( Molisch ) / according 
to Raulin also presence of zinc salts will promote the development of mould fungi. 
(2) They were infected again and left to stand for several months, whereupon a 
moderate development was observed. 
