INVESTIGATIONS ON THE MULBERRY TREE. 495 
On the 20th May, a number of leaves were collected from 
each tree for analysis ; each leaf measured on the average 9 cm. in 
length and weighed on the average (of 50) in case (A) 0.320 gram ; 
in (B) 0.304, in (C) 0.303 gram. On analysis, I obtained the follow¬ 
ing results. 
Water . 
Dry matter . 
A 
.80.82 
.19.18 
B 
80.85 
19.15 
c 
80.04 
19.96 
Organic matter (in the dry 
substance)9i.63 
90.98 
91.19 
Ash . 
.8.37 
9.02 
18.81 
Fibre . 
.13-10 
13.68 
18.11 
Fat. 
. 5-34 
4.56 
4.49 
Total carbohydrate . 
. 23.14 
22.92 
23-44 
Crude protein . 
.28.56 
23-31 
23.25 
Total nitrogen. 
•••*••••• ^ 
373 
3-72 
Albuminoid nitrogen. 
• • • .. 3-47 
3-29 
3.28 
Amido-nitrogen . 
0.44 
0.44 
Non-nitrogenous extract 
(except 
carbohydrate) .. 
26.51 
21.90 
We observe here that by liming alone, the percentage of 
woody fibre decreased from 18.10% to 13.68, while the non-intro- 
genous extract had increased from 21.90 to 26.50% ; further by 
liming and manuring with sodium nitrate and calcium sulphate» 
not only had the amount of fibre deceased from 18.11 to 13.10% 
but the proteid had increased from 23.25 to 28.56% and the fat 
from 449 to 5-34 %- 
It can not be denied that this special manuring produced 
leaves of superior nourishing quality and it remains to compare 
the effect of these different leaves upon the silk worm. (1) 
II. On the Amount of Reserve Material in the Bark 
of the Roots and Branches of 
the Mulberry Tree. 
It appeared to me of some interest to determine the extent 
to which the reserve material deposited in root and branches is 
consumed in spring time. 
On the 25th January roots about 1 cm. in diameter were col¬ 
lected whose bark was separated for analysis (a). This was re- 
(1) In September of the same year the tree B had gained in height one fourth and 
the tree A about one third compared with the control tree C. 
