5o8 SHIMADA ; 
Upon dilution with water, boiling with barium carbonate, 
evaporation of the filtrate and extraction with alcohol containing 
some ammonia, a characteristic crystallisation of leucine and 
tyrosine was obtained. The leucine was also recognised by the 
odour of amylamine on heating, while it showed tyrosine by 
Millon s reaction. 
9 
Whether also arginine, lysine, aspartic acid, glutamic acid 
and phenyl-amido-propionic acid was formed, I hope to decide 
later with larger quantities. 
For analysis the product was dried at ioo° C. 
I. 0.230 grm. gave 0.4863 grm. C 0 2 and o. 149 grm. H 2 0 . 
= 57-65 % C and 7.2 % H. 
II. 0.1636 grm. gave 0.3500 grm. C 0 2 and o. 103 grm. H 2 0 . 
= 58-34 % C and 7.0 % H. 
I. 0.5000 grm. gave after KjeldahVs method 
68.03 mg. N= 13.60^ N. 
II. 0.5000 grm. gave 67.22 mg. N= 13.45 % N. (2) 
I. i.0000 grm. substance gave after heating with a mixture 
of sodium carbonate and some potassium chlorate 0.117 grm. 
BaS 0 4 = t.6o % S. 
II. i.0000 grm. yielded by the same method 0.090 grm. 
BaS 0 4 = 1.30 % S. 
These results would correspond approximately to an albumin 
in which three hydrogen atoms have been replaced by three phenyl 
groups, when Lieb er kühn s formula is taken as a foundation. 
The product must then have been formed according to the 
following equation : — 
C 72 H 112 N 18 SO 22 4 - 3(QIi 5 OH) = C 72 H 109 (C 6 H 5 )3N 18 SO 22 +3H 2 O. 
Theory. 
Experiment. 
I. 
II. 
c 
58.69 
57.65 
5 8 -34 
H 
6.74 
7.20 
7.00 
N 
13.70 
13.60 
1345 
S 
1.74 
1.60 
1.30 
% 
0 
i 9-!3 
100.00 
(i) Another sample yielded Suzuki, of this College, 14.44 % nitrogen. 
