12 
RESEARCHES ON THE MANUFACTURE 
the nitrogen-free extract disappear, if we calculate them, as 
was done in the above, as the difference between the sum of 
the other ingredients and the total organic matter. Direct 
determinations, however, prove that the destruction of the carbo¬ 
hydrates takes place in fact to a little less extent viz. amounting 
only to 33.41 parts out of 50.28 parts applied e.g. to 66.4%. 
This apparent discrepancy is merely the result of the customary 
calculation of the so-called “ crude protein ” from the total 
nitrogen by multiplication by 6.25, which factor is too high for 
the nitrogenous bodies of miso. The increase of the ether 
extract is, as already stated, not due to a formation of fat but 
to the origin of organic acids and other substances soluble in 
ether, and the slight increase of the crude fibre is probably the 
result of the growth of new fungoid cells in the koji and the 
mixture during the fermentation. 
The course of quantitative alterations which are accomplished 
in the fermentation itself can be ascertained from our researches 
only for the last 100 days, as the first analysis was made only 
50 days after mixing the materials. In order to compare the 
results of the analysis from this point of view it is necessary to 
start with the contents of the first sample analyzed and to reduce 
the other in such proportions that their contents may accurately 
correspond to those of the first sample. A calculation of this 
kind may be based upon the contents in sodium chloride, which 
exists in the fermenting mass in a large quantity without being 
subjected to an}' conversion, the quantitative determination of 
which affords great security, because it can be carried out 
with ease and accuracy. Hence we reduced the contents of the 
2nd, 3rd and 4th samples to the proportion of common salt 
contained in the first sample (21.43 parts); for example, the 
amount of glucose in the 2nd sample, corresponding to the 
substance of the first sample is found by the equation : 23.35 : 
15.28=21.43 : X ; X=i4.o2. 
In this manner the following figures were obtained :— 
