PARASITES IN CARCASES OF BUFFALOES. 
67 
up muscular tissue into its component fibres and examining the 
preparation with the help of a microscope. We have found it 
in the striated fibres of the buffalo but only rarely and with 
considerable pains. It is therefore useless as a diagnostic feature. 
It occurs within the substance of the individual fibre without 
otherwise affecting the normal appearance of the latter. Not only 
Fig. 1.—Young Sarcocystis inside a muscular fibre of the buffalo. 
Highly magnified ; from a preparation stained with thionin. 
do the cross striations remain normal beyond the region occupied 
by the sarcocyst but between the sarcocyst and the sarcolemma or 
sheath of the muscle-fibre the striations are clearly visible. The 
youngest sarcocyst which we have observed already contained 
spores. Sporulation evidently commences at a very early stage 
after the invasion of the muscle-fibre by the germ. 
